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nanofil injection syringe  (World Precision Instruments)


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    Structured Review

    World Precision Instruments nanofil injection syringe
    Nanofil Injection Syringe, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 97/100, based on 1318 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nanofil+syringes/Sub-Microliter+Injection+System-NANOFIL-100/pm42045191-290-22-26
    Average 97 stars, based on 1318 article reviews
    nanofil injection syringe - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Injection:

    Article Title: Brain-wide input-output analysis of tuberal nucleus somatostatin neurons reveals hierarchical circuits for orchestrating feeding behavior.
    Article Snippet: .. For monosynaptic retrograde input tracing of TNSST and AgRP neurons, animals were injected with 0.3 μl equal volumemix of AAV8/ Dj-EF1a-DIO-oG-WPRE-hGHandAAV8-CAG-FLEX-TC (TVA-mCherry) by 10 μl NANOFIL syringes with 33 Gauge needles (NF33BV-2) and Micro4 syringe pump (World Precision Instruments SYS-Micro4) at 2 nl/sec into tuberal nucleus (TN) (AP: −1.6, ML: ±0.9, DV: −5.5) and arcuate nucleus (ARC) (AP:−1.5, ML: ±0.2, DV:−5.80) in SST-Cre and AgRP-Cre mice respectively. ..

    Article Title: Brain-wide input-output analysis of tuberal nucleus somatostatin neurons reveals hierarchical circuits for orchestrating feeding behavior
    Article Snippet: .. For monosynaptic retrograde input tracing of TN SST and AgRP neurons, animals were injected with 0.3 μl equal volume mix of AAV8/Dj-EF1a-DIO-oG-WPRE-hGH and AAV8-CAG-FLEX-TC (TVA-mCherry) by 10 μl NANOFIL syringes with 33 Gauge needles (NF33BV-2) and Micro4 syringe pump (World Precision Instruments SYS-Micro4) at 2 nl/sec into tuberal nucleus (TN) (AP: −1.6, ML: ±0.9, DV: −5.5) and arcuate nucleus (ARC) (AP:−1.5, ML: ±0.2, DV:−5.80) in SST-Cre and AgRP-Cre mice respectively. ..

    Article Title: Assessing the role of BNST GABA neurons in backward conditioned suppression
    Article Snippet: .. Mice were injected with AAV1-hSyn-FLEX-GCaMP6m (Addgene) unilaterally into the BNST (5 × 10 12 titer, 350 nL volume; 100 nl/min rate; +0.3 mm anteroposterior, +0.6 mm mediolateral, −4.1 mm dorsoventral coordinates from bregma) using an UltraMicroPump, Nanofil syringes, and 35-gauge needles (Micro4; World Precision Instruments, Sarasota, FL). .. For DREADD experiments, VGaT-IRES::Cre mice were bilaterally injected with either AAV8-hSyn-DIO-mCherry, AAV8-hSyn-DIO-hM4Di-mCherry, or AAV8-hSyn-DIO-hM3Dq-mCherry (Addgene) using the same coordinates as previously described.

    other:

    Article Title: Top-down control of sustained attention by the medial prefrontal cortex (mPFC)- locus coeruleus (LC) circuit during the rodent continuous performance task (rCPT)
    Article Snippet: A small hole was drilled with a 0.9 mm burr (Fine Science Tools, Foster City, CA) above each injection site ( mPFC: AP: +1.8, ML: ±0.3, DV: -1.4; LC: AP: -5.4, ML: ±0.9, DV: -3.5), and 400 nl of viral vector was injected.

    Article Title: Cortistatin neurons in the prelimbic cortex regulate seizure susceptibility in female mice via BDNF–TrkB signaling
    Article Snippet: For the TrkB knockdown experiments, mice received either AAV5-Ef1a-DIO-TrkB.DN-mCherry (UNC Vector Core) or AAV5-Ef1a-DIO-mCherry (UNC Vector Core) as a control vector.

    Mouse Assay:

    Article Title: Assessing the role of BNST GABA neurons in backward conditioned suppression
    Article Snippet: .. Mice were injected with AAV1-hSyn-FLEX-GCaMP6m (Addgene) unilaterally into the BNST (5 × 10 12 titer, 350 nL volume; 100 nl/min rate; +0.3 mm anteroposterior, +0.6 mm mediolateral, −4.1 mm dorsoventral coordinates from bregma) using an UltraMicroPump, Nanofil syringes, and 35-gauge needles (Micro4; World Precision Instruments, Sarasota, FL). .. For DREADD experiments, VGaT-IRES::Cre mice were bilaterally injected with either AAV8-hSyn-DIO-mCherry, AAV8-hSyn-DIO-hM4Di-mCherry, or AAV8-hSyn-DIO-hM3Dq-mCherry (Addgene) using the same coordinates as previously described.



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